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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022798LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022798LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022798LA01HU at dilution of 1:100

STAR Antibody

CSB-PA022798LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTAR
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAR Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6770
  • Target name
    STAR
  • Target description
    steroidogenic acute regulatory protein
  • Target synonyms
    cholesterol trafficker; mitochondrial steroid acute regulatory protein; StAR related lipid transfer (START) domain containing 1; STARD1; START domain containing 1; START domain-containing protein 1; steroid acute regulatory protein; steroidogenic acute regulator; steroidogenic acute regulatory protein, mitochondrial; testis secretory sperm-binding protein Li 241mP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49675
  • Protein Name
    Steroidogenic acute regulatory protein, mitochondrial
  • Scientific Description
    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
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Figure 1. Western blot analysis of StAR using anti-StAR antibody (A00051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
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