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Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

StAR Polyclonal Antibody

BS-3570R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSTAR
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Overview

  • Supplier
    Bioss
  • Product Name
    StAR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6770
  • Target name
    STAR
  • Target description
    steroidogenic acute regulatory protein
  • Target synonyms
    STARD1, steroidogenic acute regulatory protein, mitochondrial, START domain containing 1, START domain-containing protein 1, StAR related lipid transfer (START) domain containing 1, cholesterol trafficker, mitochondrial steroid acute regulatory protein, steroid acute regulatory protein, steroidogenic acute regulator, testis secretory sperm-binding protein Li 241mP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49675
  • Protein Name
    Steroidogenic acute regulatory protein, mitochondrial
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Xiao L, Song L, Hu J, et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019,234(9):15182-15193. doi: 10.1002/jcp.28159
    Read this paper
  • Zhang W, Zhao Y, Zhang P, et al. Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Chemosphere. 2018,194:147-157. doi: 10.1016/j.chemosphere.2017.11.164
    Read this paper
  • Shi L, Song R, Yao X, et al. Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro. Theriogenology. 2017,93:24-32. doi: 10.1016/j.theriogenology.2017.01.022
    Read this paper
  • Zhao Y, Feng Y, Zhang H, et al. Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Theriogenology. 2017,89:280-288. doi: 10.1016/j.theriogenology.2016.11.014
    Read this paper
  • Wang Y, Zhao Y, Yu S, et al. Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Toxicol Appl Pharmacol. 2016,313:149-158. doi: 10.1016/j.taap.2016.10.025
    Read this paper
  • Cook JB, Nelli SM, Neighbors MR, et al. Ethanol alters local cellular levels of (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP) independent of the adrenals in subcortical brain regions. Neuropsychopharmacology. 2014,39(8):1978-87. doi: 10.1038/npp.2014.46
    Read this paper

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Figure 1. Western blot analysis of StAR using anti-StAR antibody (A00051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
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