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Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse testis lysates probed with StAR Polyclonal Antibody (bs-3570R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

StAR Polyclonal Antibody

BS-3570R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetSTAR
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Overview

  • Supplier
    Bioss
  • Product Name
    StAR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6770
  • Target name
    STAR
  • Target description
    steroidogenic acute regulatory protein
  • Target synonyms
    STARD1, steroidogenic acute regulatory protein, mitochondrial, START domain containing 1, START domain-containing protein 1, StAR related lipid transfer (START) domain containing 1, cholesterol trafficker, mitochondrial steroid acute regulatory protein, steroid acute regulatory protein, steroidogenic acute regulator, testis secretory sperm-binding protein Li 241mP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49675
  • Protein Name
    Steroidogenic acute regulatory protein, mitochondrial
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. Xiao L et al., 2019 Jan 22, J Cell Physiol
    Read this paper
  • Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang W et al., 2018 Mar, Chemosphere
    Read this paper
  • Effects of selenium on the proliferation, apoptosis and testosterone production of sheep Leydig cells in vitro. Shi L et al., 2017 Apr 15, Theriogenology
    Read this paper
  • Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Zhao Y et al., 2017 Feb, Theriogenology
    Read this paper
  • Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang Y et al., 2016 Dec 15, Toxicol Appl Pharmacol
    Read this paper
  • Ethanol alters local cellular levels of (3alpha,5alpha)-3-hydroxypregnan-20-one (3alpha,5alpha-THP) independent of the adrenals in subcortical brain regions. Cook JB et al., 2014 Jul, Neuropsychopharmacology
    Read this paper

Datasheet

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Figure 1. Western blot analysis of StAR using anti-StAR antibody (A00051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-StAR antigen affinity purified polyclonal antibody (Catalog # A00051-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for StAR at approximately 25KD. The expected band size for StAR is at 32KD.
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