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Western Blot analysis of CoLo cells using StARD10 Polyclonal Antibody
Western Blot analysis of CoLo cells using StARD10 Polyclonal Antibody
Western Blot analysis of CoLo cells using StARD10 Polyclonal Antibody

STARD10 Antibody

CSB-PA006725
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTARD10
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Overview

  • Supplier
    Cusabio
  • Product Name
    STARD10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10809
  • Target name
    STARD10
  • Target description
    StAR related lipid transfer domain containing 10
  • Target synonyms
    antigen NY-CO-28; CGI-52; NY-CO-28; PCTP2; PCTP-L; PCTP-like protein; SDCCAG28; serologically defined colon cancer antigen 28; StAR-related lipid transfer (START) domain containing 10; stAR-related lipid transfer protein 10; START domain containing 10; START domain-containing protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y365
  • Protein Name
    START domain-containing protein 10
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP-L/STARD10 antigen affinity purified polyclonal antibody (Catalog # A10577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCTP-L/STARD10 at approximately 37 kDa. The expected band size for PCTP-L/STARD10 is at 33 kDa.
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