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STARD10 Polyclonal Antibody

CAC14512
Biomatik
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSTARD10
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Overview

  • Supplier
    Biomatik
  • Product Name
    STARD10 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10809
  • Target name
    STARD10
  • Target description
    StAR related lipid transfer domain containing 10
  • Target synonyms
    antigen NY-CO-28; CGI-52; NY-CO-28; PCTP2; PCTP-L; PCTP-like protein; SDCCAG28; serologically defined colon cancer antigen 28; StAR-related lipid transfer (START) domain containing 10; stAR-related lipid transfer protein 10; START domain containing 10; START domain-containing protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y365
  • Protein Name
    START domain-containing protein 10
  • Scientific Description
    The STARD10 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCTP-L/STARD10 using anti-PCTP-L/STARD10 antibody (A10577-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T47D whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP-L/STARD10 antigen affinity purified polyclonal antibody (Catalog # A10577-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCTP-L/STARD10 at approximately 37 kDa. The expected band size for PCTP-L/STARD10 is at 33 kDa.
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