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STAT2 antibody [C2C3], C-term

GTX103117
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSTAT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    STAT2 antibody [C2C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6773
  • Target name
    STAT2
  • Target description
    signal transducer and activator of transcription 2
  • Target synonyms
    IMD44; interferon alpha induced transcriptional activator; ISGF-3; P113; PTORCH3; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kDa; STAT113
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52630
  • Protein Name
    Signal transducer and activator of transcription 2
  • Scientific Description
    The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cells. Glykofridis IE et al., 2021 Jan 18, Elife
    Read more
  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more
  • miR-30a Remodels Subcutaneous Adipose Tissue Inflammation to Improve Insulin Sensitivity in Obesity. Koh EH et al., 2018 Dec, Diabetes
    Read more
  • Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway. Yu JS et al., 2017 Mar 24, Sci Rep
    Read more

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Figure 1. Western blot analysis of STAT2 using anti-STAT2 antibody (A01360-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT2 antigen affinity purified polyclonal antibody (Catalog # A01360-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT2 at approximately 113 kDa. The expected band size for STAT2 is at 98 kDa.
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