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Western Blot analysis of HepG2 cells using Stat2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Stat2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using Stat2 Polyclonal Antibody

STAT2 Antibody

CSB-PA004171
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSTAT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6773
  • Target name
    STAT2
  • Target description
    signal transducer and activator of transcription 2
  • Target synonyms
    IMD44, ISGF-3, P113, PTORCH3, STAT113, signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator, signal transducer and activator of transcription 2, 113kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52630
  • Protein Name
    Signal transducer and activator of transcription 2
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STAT2 using anti-STAT2 antibody (A01360-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT2 antigen affinity purified polyclonal antibody (Catalog # A01360-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT2 at approximately 113 kDa. The expected band size for STAT2 is at 98 kDa.
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Boster Bio
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ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
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TargetSTAT2
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RayBiotech
Anti-STAT2 Antibody144-63539
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ReactivityHuman, Mouse, Rat
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Biomatik
Signal Transducer And Activator Of Transcription 2 (STAT2) Polyclonal AntibodyCAU25291
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityPorcine
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Mouse brain lysates probed with Stat2 (Tyr690) Polyclonal Antibody, unconjugated (bs-3428R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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References
Bioss
Phospho-Stat2 (Tyr690) Polyclonal AntibodyBS-3428R
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Immunohistochemical staining of human skin shows moderate cytoplasmic and membranous positivity in squamous epithelial cells.
Product group Antibodies
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with STAT2 antibody [C2C3], C-term (GTX103117) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Product group Antibodies
References
GeneTex
STAT2 antibody [C2C3], C-termGTX103117
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