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Western Blot analysis of Hela cells using Stat3 Polyclonal Antibody
Western Blot analysis of Hela cells using Stat3 Polyclonal Antibody
Western Blot analysis of Hela cells using Stat3 Polyclonal Antibody

STAT3 Antibody

CSB-PA004173
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT3
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6774
  • Target name
    STAT3
  • Target description
    signal transducer and activator of transcription 3
  • Target synonyms
    acute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; HIES; signal transducer and activator of transcription 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40763
  • Protein Name
    Signal transducer and activator of transcription 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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