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STAT5B Antibody

LS-C747461
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSTAT5B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    STAT5B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 89kDa, while the observed MW by Western blot was 90kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 89kDa, while the observed MW by Western blot was 90kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6777
  • Target name
    STAT5B
  • Target description
    signal transducer and activator of transcription 5B
  • Target synonyms
    GHISID2; signal transducer and activator of transcription 5B; STAT5; transcription factor STAT5B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT5b using anti-STAT5b antibody (PB9512). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate, Lane 2: HEK293 Whole Cell Lysate, Lane 3: PANC-1 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate, Lane 5: SGC-7901 Whole Cell Lysate, Lane 6: Rat Lung Tissue Lysate. Lane 7: Rat Liver Tissue Lysate. Lane 8: Mouse Spleen Tissue Lysate. Lane 9: Mouse Spleen Tissue Lysate. Lane 10: Mouse Lung Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5b antigen affinity purified polyclonal antibody (Catalog # PB9512) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5b at approximately 90KD. The expected band size for STAT5b is at 90KD.
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Western blot All lanes: STAT5B antibody at 16 ug/ml Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa
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