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STAT5b Recombinant Antibody

BSM-52238R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT5B
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Overview

  • Supplier
    Bioss
  • Product Name
    STAT5b Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6777
  • Target name
    STAT5B
  • Target description
    signal transducer and activator of transcription 5B
  • Target synonyms
    GHISID2, STAT5, signal transducer and activator of transcription 5B, transcription factor STAT5B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51692
  • Protein Name
    Signal transducer and activator of transcription 5B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Western blot All lanes: STAT5B antibody at 16 ug/ml Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa
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Figure 1. Western blot analysis of STAT5b using anti-STAT5b antibody (PB9512). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 Whole Cell Lysate, Lane 2: HEK293 Whole Cell Lysate, Lane 3: PANC-1 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate, Lane 5: SGC-7901 Whole Cell Lysate, Lane 6: Rat Lung Tissue Lysate. Lane 7: Rat Liver Tissue Lysate. Lane 8: Mouse Spleen Tissue Lysate. Lane 9: Mouse Spleen Tissue Lysate. Lane 10: Mouse Lung Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5b antigen affinity purified polyclonal antibody (Catalog # PB9512) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5b at approximately 90KD. The expected band size for STAT5b is at 90KD.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5B antibody (GTX132113) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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