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ProductsBack/STING (TMEM173) Mouse Monoclonal Antibody [Clone ID: OTI4H1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (RC208418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173. Positive lysates LY405006 (100ug) and LC405006 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (RC208418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173. Positive lysates LY405006 (100ug) and LC405006 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM173 (RC208418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM173. Positive lysates LY405006 (100ug) and LC405006 (20ug) can be purchased separately from OriGene.

STING (TMEM173) Mouse Monoclonal Antibody [Clone ID: OTI4H1]

TA505023
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTING1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    STING (TMEM173) Mouse Monoclonal Antibody [Clone ID: OTI4H1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4H1
  • Gene ID340061
  • Target name
    STING1
  • Target description
    stimulator of interferon response cGAMP interactor 1
  • Target synonyms
    ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING, stimulator of interferon genes protein, N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner, endoplasmic reticulum IFN stimulator, endoplasmic reticulum interferon stimulator, mitochondrial mediator of IRF3 activation, stimulator of interferon protein, stimulator of interferon response cGAMP interactor-deltaC, stimulator of interferon response cGAMP interactor-deltaN, sting 1, transmembrane protein 173
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ86WV6
  • Protein Name
    Stimulator of interferon genes protein
  • Scientific Description
    TMEM173 mouse monoclonal antibody, clone OTI4H1 (formerly 4H1)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TMEM173 using anti-TMEM173 antibody (PB9513). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173 antigen affinity purified polyclonal antibody (Catalog # PB9513) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM173 at approximately 42 kDa. The expected band size for TMEM173 is at 42 kDa.
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