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Stk24 Polyclonal Antibody

CAC07485
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTK24
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Overview

  • Supplier
    Biomatik
  • Product Name
    Stk24 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8428
  • Target name
    STK24
  • Target description
    serine/threonine kinase 24
  • Target synonyms
    HEL-S-95, MST3, MST3B, STE20, STK3, serine/threonine-protein kinase 24, STE20-like kinase 3, STE20-like kinase MST3, epididymis secretory protein Li 95, mammalian STE20-like protein kinase 3, serine/threonine kinase 24 (STE20 homolog, yeast), sterile 20-like kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6E0
  • Protein Name
    Serine/threonine-protein kinase 24
  • Scientific Description
    The Stk24 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (A04112-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (Catalog # A04112-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.
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