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Western Blot analysis of 293 cells using STK33 Polyclonal Antibody
Western Blot analysis of 293 cells using STK33 Polyclonal Antibody
Western Blot analysis of 293 cells using STK33 Polyclonal Antibody

STK33 Antibody

CSB-PA004188
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTK33
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Overview

  • Supplier
    Cusabio
  • Product Name
    STK33 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65975
  • Target name
    STK33
  • Target description
    serine/threonine kinase 33
  • Target synonyms
    SPGF93, serine/threonine-protein kinase 33
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BYT3
  • Protein Name
    Serine/threonine-protein kinase 33
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STK33 using anti-STK33 antibody (M07375). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK33 antigen affinity purified monoclonal antibody (M07375) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK33 at approximately 58 kDa. The expected band size for STK33 is at 58, 50 kDa.
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