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Western blot analysis of extracts from 293 cells, using STMN3 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, using STMN3 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, using STMN3 antibody.The lane on the right is treated with the synthesized peptide.

STMN3 Rabbit Polyclonal Antibody

TA315433
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTMN3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    STMN3 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID50861
  • Target name
    STMN3
  • Target description
    stathmin 3
  • Target synonyms
    SCLIP, stathmin-3, SCG10-like protein, stathmin-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ72
  • Protein Name
    Stathmin-3
  • Scientific Description
    Rabbit polyclonal anti-STMN3 antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of STMN3 using anti-STMN3 antibody (A08661-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STMN3 antigen affinity purified polyclonal antibody (Catalog # A08661-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STMN3 at approximately 21-25 kDa. The expected band size for STMN3 is at 21 kDa.
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