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STRADB / ALS2CR2 Antibody

LS-C401232
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTRADB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    STRADB / ALS2CR2 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:15 - 1:50), WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55437
  • Target name
    STRADB
  • Target description
    STE20 related adaptor beta
  • Target synonyms
    ALS2CR2, CALS-21, ILPIP, ILPIPA, PAPK, PRO1038, STE20-related kinase adapter protein beta, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein, pseudokinase ALS2CR2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STRADB using anti-STRADB antibody (A05271-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRADB antigen affinity purified polyclonal antibody (Catalog # A05271-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STRADB at approximately 33 kDa. The expected band size for STRADB is at 47 kDa.
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