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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA227440(STRAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA227440(STRAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA227440(STRAP Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

STRAP Antibody

CSB-PA227440
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTRAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    STRAP Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11171
  • Target name
    STRAP
  • Target description
    serine/threonine kinase receptor associated protein
  • Target synonyms
    MAP activator with WD repeats; MAPK activator with WD repeats; MAWD; mitogen-activated protein kinase activator with WD repeats; PT-WD; serine-threonine kinase receptor-associated protein; unr-interacting protein; UNRIP; WD-40 repeat protein PT-WD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3F4
  • Protein Name
    Serine-threonine kinase receptor-associated protein
  • Scientific Description
    Serine-threonine kinase receptor-associated protein is an enzyme that in humans is encoded by the STRAPgene. The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic and strong nucleolar positivity in Purkinje cells.
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Figure 1. Western blot analysis of Unrip using anti-Unrip antibody (PB9407). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human Jurkat whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat brain tissue lysates Lane 6: mouse HEPA1-6 whole cell lysates Lane 7: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Unrip antigen affinity purified polyclonal antibody (Catalog # PB9407) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Unrip at approximately 39KD. The expected band size for Unrip is at 39KD.
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