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ProductsBack/Stromal interaction molecule 1 (STIM1) Mouse Monoclonal Antibody [Clone ID: CDN3H4]
Western Blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control
Western Blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control
Western Blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4) Lane 2: immunostaining with Isotype mouse IgG1 control

Stromal interaction molecule 1 (STIM1) Mouse Monoclonal Antibody [Clone ID: CDN3H4]

AM03106PU-N
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTIM1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Stromal interaction molecule 1 (STIM1) Mouse Monoclonal Antibody [Clone ID: CDN3H4]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CDN3H4
  • Gene ID6786
  • Target name
    STIM1
  • Target description
    stromal interaction molecule 1
  • Target synonyms
    D11S4896E, GOK, IMD10, STRMK, TAM, TAM1, stromal interaction molecule 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13586
  • Protein Name
    Stromal interaction molecule 1
  • Scientific Description
    Stromal interaction molecule 1 (STIM1) mouse monoclonal antibody, clone CDN3H4, Aff - Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of STIM1 using anti-STIM1 antibody (A00312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (Catalog # A00312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.
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