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ProductsBack/STS Antibody

STS Antibody

CSB-PA022883GA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTS
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Overview

  • Supplier
    Cusabio
  • Product Name
    STS Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID412
  • Target name
    STS
  • Target description
    steroid sulfatase
  • Target synonyms
    ARSC, ARSC1, ASC, ES, SSDD, XLI, steryl-sulfatase, arylsulfatase C, estrone sulfatase, steroid sulfatase (microsomal), isozyme S, steryl-sulfate sulfohydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08842
  • Protein Name
    Steryl-sulfatase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Steroid sulfatase using anti-Steroid sulfatase antibody (A01198-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Steroid sulfatase antigen affinity purified polyclonal antibody (Catalog # A01198-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Steroid sulfatase at approximately 65KD. The expected band size for Steroid sulfatase is at 65KD.
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