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Western blot analysis of extracts of various cell lines using STXBP2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using STXBP2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using STXBP2 Polyclonal Antibody at 1:1000 dilution.

STXBP2 Polyclonal Antibody

E-AB-64220
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    STXBP2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STXBP2 using anti-STXBP2 antibody (PB9820). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP2 antigen affinity purified polyclonal antibody (Catalog # PB9820) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP2 at approximately 66 kDa. The expected band size for STXBP2 is at 66 kDa.
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