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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA022915HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA022915HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA022915HA01HU at dilution of 1:100

SUB1 Antibody

CSB-PA022915HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSUB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SUB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10923
  • Target name
    SUB1
  • Target description
    SUB1 regulator of transcription
  • Target synonyms
    P15, PC4, p14, activated RNA polymerase II transcriptional coactivator p15, SUB1 homolog, transcriptional regulator, activated RNA polymerase II transcription cofactor 4, positive cofactor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53999
  • Protein Name
    Activated RNA polymerase II transcriptional coactivator p15
  • Scientific Description
    General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (A02698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC4/SUB1 antigen affinity purified polyclonal antibody (Catalog # A02698-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.
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