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SUB1 Antibody

LS-C346215
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSUB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUB1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 14kDa, while the observed MW by Western blot was 16kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), IP (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 14kDa, while the observed MW by Western blot was 16kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10923
  • Target name
    SUB1
  • Target description
    SUB1 regulator of transcription
  • Target synonyms
    activated RNA polymerase II transcription cofactor 4; activated RNA polymerase II transcriptional coactivator p15; p14; P15; PC4; positive cofactor 4; SUB1 homolog, transcriptional regulator
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (A02698-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC4/SUB1 antigen affinity purified polyclonal antibody (Catalog # A02698-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.
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