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Western blot All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 40 kDa Observed band size: 42 kDa
Western blot All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 40 kDa Observed band size: 42 kDa
Western blot All lanes: Sulfotransferase family cytosolic 2B member 1 antibody at 4ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 40 kDa Observed band size: 42 kDa

SULT2B1 Antibody

CSB-PA022943ESR2HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSULT2B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SULT2B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6820
  • Target name
    SULT2B1
  • Target description
    sulfotransferase family 2B member 1
  • Target synonyms
    alcohol sulfotransferase; ARCI14; HSST2; hydroxysteroid sulfotransferase 2; hydroxysteroid sulfotransferase SULT2B1a; ST2B1; sulfotransferase 2B1; sulfotransferase family, cytosolic, 2B, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00204
  • Protein Name
    Sulfotransferase family cytosolic 2B member 1
  • Scientific Description
    Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: A431 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.
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WB analysis of various sample lysates using GTX32904 SULT2B1 antibody. Dilution : 1:1000 Loading : 25microg per lane
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