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Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: MDA-MB-468 cell lysates probed with SULT2B1 Monoclonal Antibody, Unconjugated (bsm-51745M) at 1:8000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

SULT2B1 Monoclonal Antibody

BSM-51745M
Bioss Antibodies
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSULT2B1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SULT2B1 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:1000-2000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C11G7
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID6820
  • Target name
    SULT2B1
  • Target description
    sulfotransferase family 2B member 1
  • Target synonyms
    alcohol sulfotransferase; ARCI14; HSST2; hydroxysteroid sulfotransferase 2; hydroxysteroid sulfotransferase SULT2B1a; ST2B1; sulfotransferase 2B1; sulfotransferase family, cytosolic, 2B, member 1
  • Host
    Mouse
  • Protein IDO00204
  • Protein Name
    Sulfotransferase family cytosolic 2B member 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: A431 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.
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