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ProductsBack/Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SOD1 (Cat# RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1 (Cat# TA500495). Positive lysates [LY400160] (100ug) and [LC400160] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SOD1 (Cat# RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1 (Cat# TA500495). Positive lysates [LY400160] (100ug) and [LC400160] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SOD1 (Cat# RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1 (Cat# TA500495). Positive lysates [LY400160] (100ug) and [LC400160] (20ug) can be purchased separately from OriGene.

Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10]

CF500495
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSOD1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI8B10
  • Gene ID6647
  • Target name
    SOD1
  • Target description
    superoxide dismutase 1
  • Target synonyms
    ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer, superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP00441
  • Protein Name
    Superoxide dismutase [Cu-Zn]
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SOD1 mouse monoclonal antibody, clone OTI8B10 (formerly 8B10)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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