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Western blot analysis of various lysates using SUPT5H/SPT5 Rabbit pAb (TA382214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Western blot analysis of various lysates using SUPT5H/SPT5 Rabbit pAb (TA382214) at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 10s.
Western blot analysis of various lysates using SUPT5H/SPT5 Rabbit pAb (TA382214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

SUPT5H Rabbit Polyclonal Antibody

TA382214
OriGene
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSUPT5H
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SUPT5H Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6829
  • Target name
    SUPT5H
  • Target description
    SPT5 homolog, DSIF elongation factor subunit
  • Target synonyms
    SPT5, SPT5H, Tat-CT1, transcription elongation factor SPT5, DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, Tat-cotransactivator 1 protein, hSPT5, suppressor of Ty 5 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00267
  • Protein Name
    Transcription elongation factor SPT5
  • Scientific Description
    SUPT5H/SPT5 Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa.
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TargetSUPT5H
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TargetSUPT5H
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WB analysis of various sample lysates using GTX64922 SUPT5H antibody. Dilution : 1:1000 Loading : 25μg per lane
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TargetSUPT5H
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