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IHC-P analysis of rat lung tissue using GTX37537 Surfactant Protein D antibody. Dilution : 1:200
IHC-P analysis of rat lung tissue using GTX37537 Surfactant Protein D antibody. Dilution : 1:200
IHC-P analysis of rat lung tissue using GTX37537 Surfactant Protein D antibody. Dilution : 1:200

Surfactant Protein D antibody

GTX37537
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSFTPD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Surfactant Protein D antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-2000. IHC-P: 1:400-800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6441
  • Target name
    SFTPD
  • Target description
    surfactant protein D
  • Target synonyms
    COLEC7, PSP-D, SFTP4, SP-D, pulmonary surfactant-associated protein D, collectin-7, lung surfactant protein D, pulmonary surfactant apoprotein, surfactant-associated protein, pulmonary 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35247
  • Protein Name
    Pulmonary surfactant-associated protein D
  • Scientific Description
    The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Surfactant protein D antigen affinity purified polyclonal antibody (Catalog # PB9617) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Surfactant protein D at approximately 38 kDa. The expected band size for Surfactant protein D is at 38 kDa.
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