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Western Blot analysis of 1) Hela, 2) 293T, 3) PC-12 cells with Survivin Monoclonal Antibody
Western Blot analysis of 1) Hela, 2) 293T, 3) PC-12 cells with Survivin Monoclonal Antibody
Western Blot analysis of 1) Hela, 2) 293T, 3) PC-12 cells with Survivin Monoclonal Antibody

Survivin Monoclonal Antibody

E-AB-22112
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBIRC5
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Overview

  • Supplier
    Elabscience
  • Product Name
    Survivin Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Clone:4E2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDO15392
  • Protein Name
    Baculoviral IAP repeat-containing protein 5
  • Scientific Description
    This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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Survivin antibody detects Survivin protein at nucleus by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human breast cancer. Survivin stained by Survivin antibody (GTX100052) diluted at 1:100. Antigen Retrieval: EDTA buffer, 30 min
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Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Rabbit Anti-Survivin Polyclonal Antibody, Unconjugated (bs-0615R) at 1:200 followed by Goat Anti-Rabbit IgG, FITC conjugated (bs-0295G-FITC) used at 1:300 dilution for 40 minutes at 37°C.
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