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Survivin

ORB1945481
Biorbyt
ApplicationsImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Survivin
  • Delivery Days Customer
    10
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IHC668
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Survivin is an apoptosis inhibitor that is nearly undetectable in terminally differentiated cells, but found in most tumours including renal cell carcinoma, ovarian carcinoma, hepatocellular carcinoma, prostate carcinoma, and breast carcinoma. Survivin expression is linked to tumour progression, but not patient survival.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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