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Swine anti Human prealbumin

SwAHu/pAlb
Nordic-MUbio
TargetTTR
Product group Antibodies
1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Swine anti Human prealbumin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Precipitation assays. In immunoelectrophoresis use 2 microl human plasma or equivalent against 120 microl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 microl antiserum in 3 mm diameter center well and 2 microl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel.
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7276
  • Target name
    TTR
  • Target description
    transthyretin
  • Target synonyms
    ATTR; CTS; CTS1; epididymis luminal protein 111; HEL111; HsT2651; PALB; prealbumin, amyloidosis type I; TBPA; thyroxine-binding prealbumin; transthyretin; TTN
  • Host
    Porcine
  • Protein IDP02766
  • Protein Name
    Transthyretin
  • Source
    Transthyretin is the fastest protein in the immunoelectrophoresis pattern of human serum. It consists of four identical polypeptide chins with no carbohydrate. The molecular weight is 55,000 and the concentration in normal serum ranges from 100 to ...
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysate, Lane 2: human HCCP tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9749) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 14 kDa. The expected band size for Prealbumin is at 14 kDa.
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