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SYK Antibody

LS-C813514
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSYK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SYK Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6850
  • Target name
    SYK
  • Target description
    spleen associated tyrosine kinase
  • Target synonyms
    IMD82; p72-Syk; spleen tyrosine kinase; tyrosine-protein kinase SYK
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Flow Cytometry analysis of A431 cells using anti-SYK antibody (A00490-3). Overlay histogram showing A431 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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