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Western Blot analysis of HepG2 cells using Syk Polyclonal Antibody
Western Blot analysis of HepG2 cells using Syk Polyclonal Antibody
Western Blot analysis of HepG2 cells using Syk Polyclonal Antibody

SYK Antibody

CSB-PA004198
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSYK
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Overview

  • Supplier
    Cusabio
  • Product Name
    SYK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6850
  • Target name
    SYK
  • Target description
    spleen associated tyrosine kinase
  • Target synonyms
    IMD82, p72-Syk, tyrosine-protein kinase SYK, spleen tyrosine kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43405
  • Protein Name
    Tyrosine-protein kinase SYK
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Flow Cytometry analysis of A431 cells using anti-SYK antibody (A00490-3). Overlay histogram showing A431 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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