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ProductsBack/Synaptojanin 2 antibody [N3C1], Internal

Synaptojanin 2 antibody [N3C1], Internal

GTX115153
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSYNJ2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Synaptojanin 2 antibody [N3C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.82 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8871
  • Target name
    SYNJ2
  • Target description
    synaptojanin 2
  • Target synonyms
    INPP5H, synaptojanin-2, inositol phosphate 5'-phosphatase 2, inositol polyphosphate-5-phosphatase H, phosphoinositide 5-phosphatase, synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15056
  • Protein Name
    Synaptojanin-2
  • Scientific Description
    Synaptojanin-2 is a ubiquitously expressed inositol polyphosphate 5-phosphatase (Malecz et al., 2000 [PubMed 11084340]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SYNJ2 using anti-SYNJ2 antibody (A08206-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNJ2 antigen affinity purified polyclonal antibody (Catalog # A08206-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNJ2 at approximately 200 kDa. The expected band size for SYNJ2 is at 166 kDa.
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