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SYNJ2 Antibody

CSB-PA023017GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSYNJ2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SYNJ2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8871
  • Target name
    SYNJ2
  • Target description
    synaptojanin 2
  • Target synonyms
    INPP5H, synaptojanin-2, inositol phosphate 5'-phosphatase 2, inositol polyphosphate-5-phosphatase H, phosphoinositide 5-phosphatase, synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15056
  • Protein Name
    Synaptojanin-2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SYNJ2 using anti-SYNJ2 antibody (A08206-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNJ2 antigen affinity purified polyclonal antibody (Catalog # A08206-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNJ2 at approximately 200 kDa. The expected band size for SYNJ2 is at 166 kDa.
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