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ProductsBack/SYT16 Antibody (200-280 aa, Internal)

SYT16 Antibody (200-280 aa, Internal)

LS-C386247
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSYT16
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SYT16 Antibody (200-280 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:40000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83851
  • Target name
    SYT16
  • Target description
    synaptotagmin 16
  • Target synonyms
    CHR14SYT, SYT14L, Strep14, syt14r, synaptotagmin-16, chr14 synaptotagmin, synaptotagmin 14-like protein, synaptotagmin XIV-related protein, synaptotagmin XVI
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SYT16 using anti-SYT16 antibody (A13306-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT16 antigen affinity purified polyclonal antibody (Catalog # A13306-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYT16 at approximately 72 kDa. The expected band size for SYT16 is at 72 kDa.
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