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WB analysis of A431 (A), HeLa (B), A2058 (C), Jurkat (D) whole cell lysates using GTX55975 SYT16 antibody.
WB analysis of A431 (A), HeLa (B), A2058 (C), Jurkat (D) whole cell lysates using GTX55975 SYT16 antibody.
WB analysis of A431 (A), HeLa (B), A2058 (C), Jurkat (D) whole cell lysates using GTX55975 SYT16 antibody.

SYT16 antibody

GTX55975
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSYT16
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Overview

  • Supplier
    GeneTex
  • Product Name
    SYT16 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83851
  • Target name
    SYT16
  • Target description
    synaptotagmin 16
  • Target synonyms
    CHR14SYT, SYT14L, Strep14, syt14r, synaptotagmin-16, chr14 synaptotagmin, synaptotagmin 14-like protein, synaptotagmin XIV-related protein, synaptotagmin XVI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ17RD7
  • Protein Name
    Synaptotagmin-16
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SYT16 using anti-SYT16 antibody (A13306-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT16 antigen affinity purified polyclonal antibody (Catalog # A13306-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYT16 at approximately 72 kDa. The expected band size for SYT16 is at 72 kDa.
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