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ProductsBack/T-bet (4B10): m-IgGkappa BP-HRP Bundle

T-bet (4B10): m-IgGkappa BP-HRP Bundle

SC-520729
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetTBX21
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    T-bet (4B10): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID30009
  • Target name
    TBX21
  • Target description
    T-box transcription factor 21
  • Target synonyms
    IMD88, T-PET, T-bet, TBET, TBLYM, T-box transcription factor TBX21, T-box 21, T-box expressed in T cells, T-box protein 21, T-box transcription factor expressed in T cells, T-cell-specific T-box transcription factor T-bet, transcription factor TBLYM
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TBX21 using anti-TBX21 antibody (A00404-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX21 antigen affinity purified polyclonal antibody (Catalog # A00404-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX21 at approximately 65KD. The expected band size for TBX21 is at 58KD.
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