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The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA133615(TAB3 Antibody) at dilution 1/25. (Original magnification: x200)
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA133615(TAB3 Antibody) at dilution 1/25. (Original magnification: x200)
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA133615(TAB3 Antibody) at dilution 1/25. (Original magnification: x200)

TAB3 Antibody

CSB-PA133615
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTAB3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAB3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID257397
  • Target name
    TAB3
  • Target description
    TGF-beta activated kinase 1 (MAP3K7) binding protein 3
  • Target synonyms
    MAP3K7IP3; mitogen-activated protein kinase kinase kinase 7 interacting protein 3; NAP1; NF-kappa-B-activating protein 1; NFkB activating protein 1; TAB-3; TAK1-binding protein 3; TGF-beta activated kinase 1 and MAP3K7 binding protein 3; TGF-beta-activated kinase 1 and MAP3K7-binding protein 3; TGF-beta-activated kinase 1-binding protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N5C8
  • Protein Name
    TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
  • Scientific Description
    The product of this gene functions in the NF-kappaB signal transduction pathway. The encoded protein, and the similar and functionally redundant protein MAP3K7IP2/TAB2, forms a ternary complex with the protein kinase MAP3K7/TAK1 and either TRAF2 or TRAF6 in response to stimulation with the pro-inflammatory cytokines TNF or IL-1. Subsequent MAP3K7/TAK1 kinase activity triggers a signaling cascade leading to activation of the NF-kappaB transcription factor. The human genome contains a related pseudogene.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TAB3 using anti-TAB3 antibody (A05084-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified polyclonal antibody (Catalog # A05084-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.
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