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Tag antibodies

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Western blot analysis of extracts from HEK293 cells transfected with DYKDDDK-N terminus tagged Bad using anti-DDK rabbit polyclonal antibody.
Product group Antibodies
OriGene
Anti-DDK (FLAG) rabbit polyclonal antibodyTA100023
ApplicationsImmunoPrecipitation, Western Blot
  • SizePrice
Gel: 8%SDS-PAGE Lysate: 1/0.5 microg Lane 1-2: 1microg and 0.5 microg Fusion proteins containing the His Tag Primary antibody: TA100027 (6xHis Antibody) at dilution 1/2000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute
Product group Antibodies
OriGene
Rabbit Polyclonal Anti-6xHis AntibodyTA100027
ApplicationsWestern Blot
  • SizePrice
Gel: 8%SDS-PAGE Lysate: 0.032/0.16/0.8 microg Lane 1-3: 0.032/0.16/0.8 microg Recombinant HA tag protein Primary antibody: TA100028 (HA Tag Antibody) at dilution 1/4000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds
Product group Antibodies
OriGene
Rabbit Polyclonal Anti-HA Tag AntibodyTA100028
ApplicationsWestern Blot
  • SizePrice
Gel: 8%SDS-PAGE Lysate: 0.0016/0.008/0.032 microg Lane 1-3: 0.0016/0.008/0.032 microg Fusion proteins containing the c-Myc Tag Primary antibody: TA100029 (c-Myc Tag Antibody) at dilution 1/8000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 20 seconds
Product group Antibodies
OriGene
Rabbit Polyclonal Anti-c-Myc Tag AntibodyTA100029
ApplicationsWestern Blot
ReactivityHuman, Rat
  • SizePrice
Gel: 12%SDS-PAGE Lysate: 0.001/0.002/0.005/0.01/0.025 microg Lane 1-5: 0.001/0.002/0.005/0.01/0.025 microg Green fluorescent protein Primary antibody: TA100030 (GFP Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds
Product group Antibodies
OriGene
Rabbit Polyclonal Anti-GFP AntibodyTA100030
ApplicationsWestern Blot
  • SizePrice
Gel: 8%SDS-PAGE Lysate: 0.005/0.01/0.05/0.1 microg Lane 1-4: 0.005/0.01/0.05/0.1 microg Recombinant GST tag protein Primary antibody: TA100031 (GST Tag Antibody) at dilution 1/4000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds
Product group Antibodies
OriGene
Rabbit Polyclonal Anti-GST Tag AntibodyTA100031
ApplicationsWestern Blot
  • SizePrice
Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.
Product group Antibodies
OriGene
Anti-Myc and Anti-DDK monoclonal antibodiesTA150014
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • SizePrice
Human ELISA of DDK-fused protein level by using anti-DDK antibody in nuclear and cytoplasmic extracts from the peripheral blood mononuclear cells of individuals vaccinated with the BCG vaccine. Humanhttp://www.jbc.org/cgi/reprint/286/52/44799Human
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, BiotinTA150015
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
  • SizePrice
Normal CHO cells (black line) or CHO cells stably expressing FLAG-tagged CB1 (red line) were fixed with 2% formaldehyde for 20 min at RT, stained with PE-labeled 4C5 anti-DDK antibody and analyzed by flow cytometry.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE)TA150020
ApplicationsFlow Cytometry
  • SizePrice
CHO cells were tranfected with BAF57-Myc/DDK plasmid (OriGene Cat# RC209444). The cells were fixed with 2% formaldehyde for 20 min, and permeabilized with 0.1% saponin in PBS for 10 min. The cells were then incubated with DyLight488-labeled DDK antibodies diluted 1:50 in PBS/0.1% saponin for 20 min. The cells were then washed and observed under a fluorescent microscope. Please note the nucleus staining pattern for BAF57.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488TA150021
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 550TA150022
ApplicationsImmunoFluorescence
  • SizePrice
Hela cells transiently transfected with Paxillin-GFP (RG225997) were detached and seeded onto 0.2% gelatin coated coverslip. After 24 hours incubation, the cells were fixed with 4% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with Dylight594-labeled tGFP antibodies (TA150024) and DAPI for nucleus counter staining. (a: Paxillin-GFP; b: anti-tGFP-DyLight594; c: Merged)
Product group Antibodies
OriGene
Mouse monoclonal tGFP antibody (clone OTI2H8), DyLight 594 conjugatedTA150024
ApplicationsImmunoFluorescence
  • SizePrice
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