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Tag antibodies

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Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.
Product group Antibodies
OriGene
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic BeadsTA150039S
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.
Product group Antibodies
OriGene
Mouse monoclonal turboGFP antibody, clone OTI2H8TA150041
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • SizePrice
Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.
Product group Antibodies
OriGene
Mouse monoclonal turboGFP antibody, clone OTI2H8TA150041S
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • SizePrice
Immunoprecipitation was performed with lysates of HEK293T cells overexpressing a GFP-tagged vector (Cat# PS100010) or DDK-tagged PON3 (Cat# LY400339). Please see datasheet for details.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic BeadsTA150042
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoprecipitation was performed with lysates of HEK293T cells overexpressing a GFP-tagged vector (Cat# PS100010) or DDK-tagged PON3 (Cat# LY400339). Please see datasheet for details.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic BeadsTA150042S
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
HEK293T cells were transfected with pCMV6-AC-tGFPvector (Cat# PS100010, Lane 1) or pCMV6-Entry-MCR2 (Myc-DDK-tagged ORF clone of human MCR2 gene, Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tGFP-HRP (1:1000).
Product group Antibodies
OriGene
Mouse monoclonal tGFP antibody (clone OTI2H8), HRP conjugatedTA150043
ApplicationsWestern Blot
  • SizePrice
Immunoprecipitation (IP) experiments were performed with negative control lysate (Cat# LY40001) generated by transfecting HEK293T cells with pCMV-entry empty vector (Cat# PS100001), and with overexpression lysate (Cat# LY400339) generated by transfecting TrueORF Gold cDNA clone myc-DDK tagged PON3 (Cat# RC210019) to HEK293T cells. Please see datasheet for details.
Product group Antibodies
OriGene
Mouse Monoclonal Myc Antibody, Clone 9E10, Magnetic BeadsTA150044
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoprecipitation (IP) experiments were performed with negative control lysate (Cat# LY40001) generated by transfecting HEK293T cells with pCMV-entry empty vector (Cat# PS100001), and with overexpression lysate (Cat# LY400339) generated by transfecting TrueORF Gold cDNA clone myc-DDK tagged PON3 (Cat# RC210019) to HEK293T cells. Please see datasheet for details.
Product group Antibodies
OriGene
Mouse Monoclonal Myc Antibody, Clone 9E10, Magnetic BeadsTA150044S
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).
Product group Antibodies
OriGene
Mouse monoclonal eGFP antibody, clone OTI5A2TA150052
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).
Product group Antibodies
OriGene
Mouse monoclonal eGFP antibody, clone OTI5A2TA150052S
ApplicationsWestern Blot
  • SizePrice
HEK293T cells were transfected with pCMV6-AC-GFPvector (control plasmid, Lane 1) or Myc-DDK-tagged ORF clone of human MC2R gene (Cat# RC210483, Lane 2) plasmids for 48 hrs. Eqivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Myc tag-HRP. Conditio: 1:1000 dilution.
Product group Antibodies
OriGene
9E10, Anti-Myc tag monoclonal antibody, HRPTA150054
ApplicationsWestern Blot
  • SizePrice
Anti-tRFP immunoblot analysis of HEK293T over-expression lysates containing recombinant tRFP, TurboFP602 TurboFP635 fluorescence proteins at 1:2000 dilution.
Product group Antibodies
OriGene
Mouse monoclonal turboRFP antibody, clone OTI2D9TA150061
ApplicationsWestern Blot
  • SizePrice
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