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Western blot analysis of extracts of various cell lines using TAS2R10 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using TAS2R10 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using TAS2R10 Polyclonal Antibody at dilution of 1:1000.

TAS2R10 Polyclonal Antibody

E-AB-65221
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TAS2R10 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAS2R10 using anti-TAS2R10 antibody (A10959-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS2R10 antigen affinity purified polyclonal antibody (Catalog # A10959-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAS2R10 at approximately 38 kDa. The expected band size for TAS2R10 is at 35 kDa.
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