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TAX1BP1 Antibody (YA2248)

HY-P82503
MedChem Express
TargetTAX1BP1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TAX1BP1 Antibody (YA2248)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8887
  • Target name
    TAX1BP1
  • Target description
    Tax1 binding protein 1
  • Target synonyms
    CALCOCO3; T6BP; Tax1 (human T-cell leukemia virus type I) binding protein 1; tax1-binding protein 1; TRAF6-binding protein; TXBP151
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TAX1BP1 Antibody (YA2248) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2248), targeting TAX1BP1, with a predicted molecular weight of 91 kDa (observed band size: 91 kDa). TAX1BP1 Antibody (YA2248) can be used for WB, IHC-P experiment in human background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa.
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