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ProductsBack/TAX1BP1 Recombinant Antibody, AbBy Fluor-555 Conjugated

TAX1BP1 Recombinant Antibody, AbBy Fluor-555 Conjugated

BSM-62170R-BF555
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetTAX1BP1
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Overview

  • Supplier
    Bioss
  • Product Name
    TAX1BP1 Recombinant Antibody, AbBy Fluor-555 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID8887
  • Target name
    TAX1BP1
  • Target description
    Tax1 binding protein 1
  • Target synonyms
    CALCOCO3, T6BP, TXBP151, tax1-binding protein 1, TRAF6-binding protein, Tax1 (human T-cell leukemia virus type I) binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VP1
  • Protein Name
    Tax1-binding protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa.
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