
TCF3 / E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TCF3 stained by TCF3 / E2A antibody (GTX109885) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TCF3 / E2A antibody
GTX109885
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTCF3
Overview
- SupplierGeneTex
- Product NameTCF3 / E2A antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.19 mg/ml
- ConjugateUnconjugated
- Gene ID6929
- Target nameTCF3
- Target descriptiontranscription factor 3
- Target synonymsAGM8, AGM8A, AGM8B, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75, transcription factor E2-alpha, E2A-HLF fusion transcript protein, NOL1-TCF3 fusion, VDR interacting repressor, class B basic helix-loop-helix protein 21, helix-loop-helix protein HE47, immunoglobulin transcription factor 1, kappa-E2-binding factor, negative vitamin D response element-binding protein, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), transcription factor ITF-1, vitamin D receptor-interacting repressor
- HostRabbit
- IsotypeIgG
- Protein IDP15923
- Protein NameTranscription factor E2-alpha
- Scientific DescriptionThe TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203


![TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments. IP samples: Jurkat whole cell extract A. 35 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 / E2A antibody (GTX109885) 7.5 % SDS-PAGE The immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (GTX109885) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments. IP samples: Jurkat whole cell extract A. 35 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 / E2A antibody (GTX109885) 7.5 % SDS-PAGE The immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (GTX109885) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX109885/GTX109885_40912_IP_w_23060500_279.webp)







![FACS analysis of A549 cells using GTX83355 TCF3 / E2A antibody [6B8]. Green : TCF3 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX83355/GTX83355_20170912_FACS_w_23061322_378.webp)

