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Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TDRD3 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 84 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TDRD3 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 84 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: TDRD3 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 84 kDa Observed band size: 74 kDa

TDRD3 Antibody

CSB-PA863996LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTDRD3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TDRD3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81550
  • Target name
    TDRD3
  • Target description
    tudor domain containing 3
  • Target synonyms
    tudor domain-containing protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H7E2
  • Protein Name
    Tudor domain-containing protein 3
  • Scientific Description
    Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & the Golgi apparatus.
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Figure 1. Western blot analysis of TDRD3 using anti-TDRD3 antibody (A08978). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD3 antigen affinity purified polyclonal antibody (Catalog # A08978) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for TDRD3 at approximately 65-73KD. The expected band size for TDRD3 is at 73KD.
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