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ICC/IF analysis of COS7 cells using GTX87949 TENS3 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of COS7 cells using GTX87949 TENS3 antibody. The picture on the right is blocked with the synthesized peptide.
ICC/IF analysis of COS7 cells using GTX87949 TENS3 antibody. The picture on the right is blocked with the synthesized peptide.

TENS3 antibody

GTX87949
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTNS3
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Overview

  • Supplier
    GeneTex
  • Product Name
    TENS3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100~1:500. IHC-P: 1:50~1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64759
  • Target name
    TNS3
  • Target description
    tensin 3
  • Target synonyms
    TEM6; TENS1; tensin-3; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing protein 1; thyroid specific PTB domain protein; tumor endothelial marker 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68CZ2
  • Protein Name
    Tensin-3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNS3 using anti-TNS3 antibody (A07345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (Catalog # A07345-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.
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