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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA023386EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA023386EA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA023386EA01HU at dilution of 1:100

TERF1 Antibody

CSB-PA023386EA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTERF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TERF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7013
  • Target name
    TERF1
  • Target description
    telomeric repeat binding factor 1
  • Target synonyms
    hTRF1-AS; NIMA-interacting protein 2; PIN2; telomeric protein Pin2/TRF1; telomeric repeat binding factor (NIMA-interacting) 1; telomeric repeat-binding factor 1; TRBF1; TRF; TRF1; TTAGGG repeat-binding factor 1; t-TRF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54274
  • Protein Name
    Telomeric repeat-binding factor 1
  • Scientific Description
    Binds the telomeric double-stranded 5-TTAGGG-3 repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5-TTAGGG-3 repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (Catalog # A02474-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.
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