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TERF1 Antibody (YA3239)

HY-P83494
MedChem Express
TargetTERF1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    TERF1 Antibody (YA3239)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7013
  • Target name
    TERF1
  • Target description
    telomeric repeat binding factor 1
  • Target synonyms
    hTRF1-AS; NIMA-interacting protein 2; PIN2; telomeric protein Pin2/TRF1; telomeric repeat binding factor (NIMA-interacting) 1; telomeric repeat-binding factor 1; TRBF1; TRF; TRF1; TTAGGG repeat-binding factor 1; t-TRF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    TERF1 Antibody (YA3239) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3239), targeting TERF1, with a predicted molecular weight of 50 kDa (observed band size: 50 kDa). TERF1 Antibody (YA3239) can be used for WB, ICC/IF experiment in human, mouse background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRF1/TERF1 using anti-TRF1/TERF1 antibody (A02474-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF1/TERF1 antigen affinity purified polyclonal antibody (Catalog # A02474-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF1/TERF1 at approximately 55 kDa. The expected band size for TRF1/TERF1 is at 50 kDa.
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