TMB Substrate

GeneTex

Tetramethylbenzidine (TMB) substrate

9

Following reaction with peroxidase, a blue reaction product forms that may be read at 370 nm or between 620 and 655 nm. For end-point assays, the reaction can be stopped by the addition of a volume of 1 M or 2 M HCl, or 0.5 M H2SO4, equal to the volume of the substrate reaction in the well. The resulting yellow end product, which is stable for at least one hour, can then be read at 450 nm. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.

ELISA

Research Use Only

Liquid

3-3,5,5-tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems. It is recommended for ELISA procedures.

2°C to 8°C

41116133