TMB Substrate

GeneTex

TMB Substrate

9

Allow the substrate to equilibrate at room temperature (~25C) prior to use. End-Point Assays: (1) Add 100 microL of equilibrated substrate solution to each microwell. (2) Allow the substrate reaction to develop a blue soluble reaction by incubating for the optimal time determined for the assay. (3) The reaction should be stopped by using a 450 nm or 650 nm stop solution. (4) Measure the absorbance at 450 nm or 650 nm of each microwell within 1 hour. Kinetic Assays: (1) Add 100 microL of equilibrated substrate solution to each microtiter plate well. (2) Measure the absorbance at 650 nm at multiple time points. (3) Calculate the rate of absorbance change for each microwell.

ELISA

Research Use Only

Liquid

GeneTex TMB substrate is supplied as a one-component- ready-to-use solution. It is colorless or very light yellow in appearance. When TMB reacts with peroxidase, a blue liquid is yielded (oxidized product). The reaction can be terminated using 0.6N HCl. GeneTex TMB substrate is not recommended for membrane or immunohistochemistry applications where a colored precipitate is desired.

2°C to 8°C

41116133