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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA867154LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA867154LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA867154LA01HU at dilution of 1:100

TFB2M Antibody

CSB-PA867154LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTFB2M
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Overview

  • Supplier
    Cusabio
  • Product Name
    TFB2M Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64216
  • Target name
    TFB2M
  • Target description
    transcription factor B2, mitochondrial
  • Target synonyms
    dimethyladenosine transferase 2, mitochondrial; HCV NS5A-transactivated protein 5; hepatitis C virus NS5A-transactivated protein 5; Hkp1; h-mtTFB; h-mtTFB2; hTFB2M; mitochondrial 12S rRNA dimethylase 2; mitochondrial transcription factor B2; mtTFB2; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H5Q4
  • Protein Name
    Dimethyladenosine transferase 2, mitochondrial
  • Scientific Description
    S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TFB2M using anti-TFB2M antibody (A05679-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFB2M antigen affinity purified polyclonal antibody (Catalog # A05679-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFB2M at approximately 38 kDa. The expected band size for TFB2M is at 45 kDa.
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