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ProductsBack/TFF1 / pS2 Antibody (aa25-84)

TFF1 / pS2 Antibody (aa25-84)

LS-C407735
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTFF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TFF1 / pS2 Antibody (aa25-84)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (0.1 - 0.5 µg/ml), WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7031
  • Target name
    TFF1
  • Target description
    trefoil factor 1
  • Target synonyms
    BCEI, D21S21, HP1.A, HPS2, pNR-2, pS2, trefoil factor 1, breast cancer estrogen-inducible protein, breast cancer estrogen-inducible sequence, gastrointestinal trefoil protein pS2, polypeptide P1.A, protein pS2
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TFF1 using anti-TFF1 antibody (A01391-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF1 antigen affinity purified polyclonal antibody (Catalog # A01391-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFF1 at approximately 12 kDa. The expected band size for TFF1 is at 9 kDa.
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